Intended Use

For Research Use Only. Not for use in diagnostic procedures.

The MouseTRAP™ test is a solid phase immunofi xed enzyme activity assay for the determination of osteoclastderived tartrate-resistant acid phosphatase form 5b (TRACP 5b) in mouse serum samples .

Summary and Explanation

TRAP is expressed in bone-resorbing osteoclasts and secreted into the circulation. Thus, serum TRAP has been considered as a potentially useful marker of bone resorption rate. However, several problems have prevented the development of specifi c TRAP assays including the presence of other interfering acid phosphatases in the blood circulation.

MouseTRAP™ Assay is specifi c for circulating TRACP 5b derived from mouse osteoclasts. And even more,MouseTRAP™ Assay detects ONLY the active TRACP molecules that are freshly liberated into the circulation.It does NOT detect old circulating inactive TRACP molecules or fragments. Thus, MouseTRAP Assay gives an accurate estimation of the amount of bone resorption rate at the time of sample collection.

Method Description

MouseTRAP™ Assay uses a polyclonal antibody prepared using generated recombinant mouse TRACP (2) as antigen.

In the test, the antibody is incubated in anti-rabbit IgG-coated microtiter wells. After washing, standard, control, and serum samples are incubated in the wells, and bound TRACP 5b activity is determined with a chromogenic substrate

to develop color. The reaction is stopped, and the absorbance of the reaction mixture is read in a microtiter plate reader, color intensity being directly proportional to the amount and activity of TRACP 5b present in the sample.

Warnings and Precautions

The MouseTRAP™ Assay is for research use only and is not for internal use in humans or animals. This product must be used strictly in accordance with the instructions set out in the Package Insert. IDS Limited will not be held

responsible for any loss or damage (except as required by statute) howsoever caused, arising out of non-compliance with the instructions provided.

CAUTION: this kit contains material of animal origin. Handle kit reagents as if capable of transmitting an infectious agent.Appropriate precautions and good laboratory practices must be used in the storage, handling and disposal of the

kit reagents. Disposal of kit reagents should be in accordance with local regulations.